Additional data should be considered for a more accurate adjustment.Īpproximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). The entire western blotting workflow consists of several individual steps, each of which is critical to producing high. All from a starting mixture of proteins extracted from cells or tissues. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights. 2.5 μl per well for general Western transferring. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. Refer to the Blu11 (BlueAQUA) Prestained Protein Ladder patterns in various electrophoresis conditions:ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). A western blot provides a readout of differences in protein expression levels, for example changes in protein levels over a certain time period, differences in knock-out versus wild type cell lines, and altered expression in patient samples. Guide for Molecular Weight Estimation (kDa) Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. The Blu11 Prestained Protein Ladder (BlueAQUA) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. New England Biolabs offers a selection of highly pure protein standards. The 11 recombinant proteins are covalently coupled with blue chromophore, and 2 reference bands at 25 kDa and 72 kDa serve as reference bands. The Blu11 Prestained Protein Ladder (BlueAQUA) is a combination of 11 pre-stained proteins with molecular weights from 10 to 180 kDa.
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